#!/usr/bin/env fslpython """Test the standard recipe for fixing a broken orientation, from "The labels in FSLView are wrong or missing and no conversion to NIfTI can fix this", on this page: https://fsl.fmrib.ox.ac.uk/fsl/fslwiki/Orientation%20Explained If this really cannot be fixed at the conversion stage, then it is possible to fix by the follow (only to be attempted after reading the following section on background information on NIfTI orientation): fslorient -deleteorient imagename ; fslswapdim imagename a b c imagename ; fslorient -setqformcode 1 imagename ; where a b c need to be chosen, usually by trial and error, so that the image after this is in the same orientation (as viewed in FSLView) as the MNI152 images. However, unless you have some way of telling left from right in the image (e.g. by a vitamin capsule or clearly known structural asymmetry) this method can easily result in an incorrect left-right labelling and so should not be attempted. The recipe involves: 1. resetting the image affines 2. transposing the data array until it is in a RAS orientation 3. Enabling the qform affine which, after step 1, should be a diagonal matrix with pixdims as scaling parameters. Specifically, the recipe is as follows: # 1. clear the sform/qform affines fslorient -deleteorient image # 2. rotate the data into RAS orientation (exact # command depends on original data orientation) fslswapdim image x z y image # 3. reset the qform code. The qform should be # a diagonal matrix, and the image data array # should be in RAS orientation, so after this # step the image should be labelled correctly. fslorient -setqformcode 1 image This recipe makes some key assumptions: - fslorient -deleteorient (step 1) deleting/ clearing the affines - the qform must be reset to be a scaling matrix. - fslswapdim (step 2) not touching the affines when the s/qform codes are 0 These are required so that, in step 3, the qform is a diagonal matrix with correct scaling paramaters. Some bugs were introduced in FSL 6.0.0 (fixed in FSL 6.0.6) which broke the above assumptions: - fslorient -deleteorient was not clearing the original affines - fslswapdim was applying the rotations/flips to both the data, and to the s/qforms, even if the s/qform codes were set to 0. """ import numpy as np import nibabel as nib import os import subprocess as sp import shlex import sys import traceback def sprun(cmd): sp.run(shlex.split(cmd), check=True) # Test the original recipe for fixing the # orientation of an image, as described above def test_fix_orient(): data = np.random.random((10, 10, 10)) orig_qform = np.array([[2, 0, 0, 10], [0, 2, 0, -20], [0, 0, 2, 50], [0, 0, 0, 1]]) image = nib.Nifti1Image(data, np.eye(4)) image.set_sform(np.eye(4), 0) image.set_qform(orig_qform, 1) image.to_filename('orig.nii.gz') sprun('imcp orig cleared') sprun('fslorient -deleteorient cleared') sprun('fslswapdim cleared x z y swapped') sprun('imcp swapped fixed') sprun('fslorient -setqformcode 1 fixed') fixed = nib.load('fixed.nii.gz') new_data = fixed.get_fdata() new_qform, qcode = fixed.get_qform(coded=True) _, scode = fixed.get_sform(coded=True) # new qform should be a scaling matrix (not # necessarily equivalent to the original assert qcode == 1 assert scode == 0 assert np.all(np.isclose(new_qform, np.diag([2, 2, 2, 1]))) assert np.all(np.isclose(new_data, data.transpose((0, 2, 1)))) # An alternate strategy which involves # explicitly clobbering the qform def test_fix_orient_alternate(): # original qform data = np.random.random((10, 10, 10)) orig_qform = np.array([[2, 0, 0, 10], [0, 2, 0, -20], [0, 0, 2, 50], [0, 0, 0, 1]]) image = nib.Nifti1Image(data, np.eye(4)) image.set_sform(np.eye(4), 0) image.set_qform(orig_qform, 1) image.to_filename('orig.nii.gz') sprun('imcp orig cleared') sprun('fslorient -deleteorient cleared') sprun('fslswapdim cleared x z y swapped') sprun('imcp swapped fixed1') sprun('fslorient -setqform 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1 fixed1') sprun('imcp fixed1 fixed') sprun('fslorient -setqformcode 1 fixed') fixed = nib.load('fixed.nii.gz') new_data = fixed.get_fdata() new_qform, qcode = fixed.get_qform(coded=True) _, scode = fixed.get_sform(coded=True) # new qform should be a scaling matrix (not # necessarily equivalent to the original assert qcode == 1 assert scode == 0 assert np.all(np.isclose(new_qform, np.diag([2, 2, 2, 1]))) assert np.all(np.isclose(new_data, data.transpose((0, 2, 1)))) if __name__ == '__main__': tests = [test_fix_orient, test_fix_orient_alternate] result = 0 for test in tests: try: os.chdir(sys.argv[1]) test() print(f'\nTest {test.__name__} PASSED') except Exception as e: print(f'\nTest {test.__name__} FAILED') traceback.print_exc() result = 1 sys.exit(result)